LOCUS       sequence-127735-         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked SpHIS5 marker, integration into S. cerevisiae X-4 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2226
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      99..288
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     protein_bind    336..369
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        424..767
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 cttatttaat aataaaaatc ataaatcata agaaattcgc ttatttagaa gtgtcaacaa
       61 cgtatctacc aacggaatgc gtgcgatcgc gtgcattcat ccgctctaac cgaaaaggaa
      121 ggagttagac aacctgaagt ctaggtccct atttattttt ttatagttat gttagtatta
      181 agaacgttat ttatatttca aatttttctt ttttttctgt acagacgcgt gtacgcatgt
      241 aacattatac tgaaaacctt gcttgagaag gttttgggac gctcgaagat cgcgtcagct
      301 gaagcttcgt acgctgcagg tcgacaaccc ttaatataac ttcgtataat gtatgctata
      361 cgaagttatt aggtctagag atctgtttag cttgcctcgt ccccgccggg tcacccggcc
      421 agcgacatgg aggcccagaa taccctcctt gacagtcttg acgtgcgcag ctcaggggca
      481 tgatgtgact gtcgcccgta catttagccc atacatcccc atgtataatc atttgcatcc
      541 atacattttg atggccgcac ggcgcgaagc aaaaattacg gctcctcgct gcagacctgc
      601 gagcagggaa acgctcccct cacagacgcg ttgaattgtc cccacgccgc gcccctgtag
      661 agaaatataa aaggttagga tttgccactg aggttcttct ttcatatact tccttttaaa
      721 atcttgctag gatacagttc tcacatcaca tccgaacata aacaaccatg ggtaggaggg
      781 cttttgtaga aagaaatacg aacgaaacga aaatcagcgt tgccatcgct ttggacaaag
      841 ctcccttacc tgaagagtcg aattttattg atgaacttat aacttccaag catgcaaacc
      901 aaaagggaga acaagtaatc caagtagaca cgggaattgg attcttggat cacatgtatc
      961 atgcactggc taaa
//
