LOCUS       sequence-127737-         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG304GAL-ccdB-Cerulean
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        2..65
                     /gene="bla"
                     /label=AmpR promoter
     CDS             250..628
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(284..303)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
       61 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      121 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      181 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      241 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      301 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      361 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      421 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      481 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      541 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      601 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      661 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      721 tgcgctcgg
//
