LOCUS       sequence-127743-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  One half component of the optimized Cre recombinase dependent on GFP
            (CRE-DOG OPT) for rAAV production and expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Tang JC, Rudolph S, Dhande OS, Abraira VE, Choi S, Lapan SW, Drew 
            IR, Drokhlyansky E, Huberman AD, Regehr WG, Cepko CL
  TITLE     Cell type-specific manipulation with GFP-dependent Cre recombinase.
  JOURNAL   Nat Neurosci. 2015 Sep;18(9):1334-41. doi: 10.1038/nn.4081. Epub 
            2015 Aug 10.
  PUBMED    26258682
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.4081"; 
            journalName: "Nat Neurosci"; date: "2015-09"; volume: "18"; issue: 
            "9"; pages: "1334-41"
COMMENT     Sequence Label: pAAV-EF1a-N-CretrcintG
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             75..95
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     gap             402..791
                     /estimated_length=390
     primer_bind     complement(872..892)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 tttttcttcc atttcaggtg tcgtgaggta ccggatccgc caccatggat aaagcggaat
       61 taattcccga gcctccaaaa aagaagagaa aggtcgaatt gggtaccacc tctgatgaag
      121 tcaggaagaa cctgatggac atgttcaggg acaggcaggc cttctctgaa cacacctgga
      181 agatgctcct gtctgtgtgc agatcctggg ctgcctggtg caagctgaac ctcgtcgaaa
      241 gctatcgcaa ggccagcaac aaggcctact ttgagtggac catcgaagcc agggacctct
      301 ccctgctggg aagccacgtg cgcaaggcca cctaccagac ctacgccccc atcctgtacg
      361 ctagcggagg aggtggtgga ggtggaggtg gaggtcaatt gnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn ntaagaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa
      841 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
      901 ctgctttaat gcctttgtat catgctattg
//
