LOCUS       sequence-127744-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  One half component of the optimized Cre recombinase dependent on GFP
            (CRE-DOG OPT) for rAAV production and expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Tang JC, Rudolph S, Dhande OS, Abraira VE, Choi S, Lapan SW, Drew 
            IR, Drokhlyansky E, Huberman AD, Regehr WG, Cepko CL
  TITLE     Cell type-specific manipulation with GFP-dependent Cre recombinase.
  JOURNAL   Nat Neurosci. 2015 Sep;18(9):1334-41. doi: 10.1038/nn.4081. Epub 
            2015 Aug 10.
  PUBMED    26258682
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.4081"; 
            journalName: "Nat Neurosci"; date: "2015-09"; volume: "18"; issue: 
            "9"; pages: "1334-41"
COMMENT     Sequence Label: pAAV-EF1a-N-CretrcintG
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    216..804
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             322..333
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     731..751
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 aggttgtctt cccaacttgc cccttgctcc ataccacccc cctccacccc ataatattat
       61 agaaggacac ctagtcagac aaaatgatgc aacttaattt tattaggaca aggctggtgg
      121 gcactggagt ggcaacttcc agggccagga gaggcactgg ggaggggtca cagggatgcc
      181 acccgtagat ctctcgagca gcgctcggta tcgatgcggg gaggcggccc aaagggagat
      241 ccgactcgtc tgagggcgaa ggcgaagacg cggaagaggc cgcagagccg gcagcaggcc
      301 gcgggaagga aggtccgctg gattgagggc cgaagggacg tagcagaagg acgtcccgcg
      361 cagaatccag gtggcaacat aggcgagcag ccaaggaaag gacgatgatt tccccgacaa
      421 caccacggaa ttgtcagtgc ccaacagccg agcccctgtc cagcagcggg caaggcaggc
      481 ggcgatgagt tccgccgtgg caatagggag ggggaaagcg aaagtcccgg aaaggagctg
      541 acaggtggtg gcaatgcccc aaccagtggg ggttgcgtca gcaaacacag tgcacaccac
      601 gccacgttgc ctgacaacgg gccacaactc ctcataaaga gacagcaacc aggatttata
      661 caaggaggag aaaatgaaag ccatacggga agcaatagca tgatacaaag gcattaaagc
      721 agcgtatcca catagcgtaa aaggagcaac atagttaaga ataccagtca atctttcaca
      781 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattctt a
//
