LOCUS       sequence-127752-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses eZinCh-2 Zn2+ FRET sensor in the cytosol of mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Hessels AM, Chabosseau P, Bakker MH, Engelen W, Rutter GA, Taylor 
            KM, Merkx M
  TITLE     eZinCh-2: A Versatile, Genetically Encoded FRET Sensor for Cytosolic
            and Intraorganelle Zn(2+) Imaging.
  JOURNAL   ACS Chem Biol. 2015 Sep 18;10(9):2126-34. doi: 
            10.1021/acschembio.5b00211. Epub 2015 Jul 20.
  PUBMED    26151333
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.5b00211"; journalName: "ACS Chem Biol"; date: 
            "2015-09-18- 18"; volume: "10"; issue: "9"; pages: "2126-34"
COMMENT     Sequence Label: peZinCh-2
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(149..859,860..862,863..865))
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSHLCKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(191..212)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     540..559
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     799..820
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
       61 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
      121 attatgatct agagtcgcgg ccgctttact tgtacagctc gtccatgccg agagtgatcc
      181 cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc
      241 acaggtggga ctggtagctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga
      301 tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc
      361 ggatcttgaa gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt
      421 ggctgttgta gttgtactca agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga
      481 tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg
      541 tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca
      601 tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcgggcg aagcacatca
      661 ggccgtagcc gaaggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc
      721 agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc
      781 tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga
      841 acagctcctc gcccttgctc accatgggta ccgagccccg cggacccccg
//
