LOCUS       sequence-127755-        1074 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses ER-eZinCh-2 Zn2+ FRET sensor in the ER of mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Hessels AM, Chabosseau P, Bakker MH, Engelen W, Rutter GA, Taylor 
            KM, Merkx M
  TITLE     eZinCh-2: A Versatile, Genetically Encoded FRET Sensor for Cytosolic
            and Intraorganelle Zn(2+) Imaging.
  JOURNAL   ACS Chem Biol. 2015 Sep 18;10(9):2126-34. doi: 
            10.1021/acschembio.5b00211. Epub 2015 Jul 20.
  PUBMED    26151333
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.5b00211"; journalName: "ACS Chem Biol"; date: 
            "2015-09-18- 18"; volume: "10"; issue: "9"; pages: "2126-34"
COMMENT     Sequence Label: pER-eZinCh-2
FEATURES             Location/Qualifiers
     source          1..1074
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     99..170
                     /codon_start=1
                     /product="signal sequence of human preproinsulin"
                     /label=insulin signal sequence
                     /translation="MALWMRLLPLLALLALWGPDPAAA"
     CDS             join(177..179,180..182,183..893)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSHLCKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(222..243)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(483..502)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     830..851
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcat ggccctgtgg atgcgcctcc
      121 tgcccctgct ggcgctgctg gccctctggg gacctgaccc agccgcagcc gaattcatgg
      181 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
      241 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
      301 agctgaccct gaagttcatc tgcaccaccg gtaagctgcc cgtgccctgg cccaccctcg
      361 tgaccaccct gacctggggc gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc
      421 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
      481 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
      541 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
      601 tggagtacaa cgccatcagc gacaacgtct atatcaccgc cgacaagcag aagaacggca
      661 tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
      721 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
      781 tgagcaccca gtcccacctg tgcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
      841 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtccggag
      901 gcggcgagct cattcgtggc ggatccggcg gaagcggcgg atccggcggt agcggcggat
      961 ccggcggctc cggcggatcc ggcggcagcg gcggatccgg tggaagcggt ggatccggtg
     1021 gtagcggtgg atccggtgga agcggtggat ccggtggtag cggtggatcc gggg
//
