LOCUS       sequence-127757-        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses ER-eZinCh-2 Zn2+ FRET sensor in the ER of mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Hessels AM, Chabosseau P, Bakker MH, Engelen W, Rutter GA, Taylor 
            KM, Merkx M
  TITLE     eZinCh-2: A Versatile, Genetically Encoded FRET Sensor for Cytosolic
            and Intraorganelle Zn(2+) Imaging.
  JOURNAL   ACS Chem Biol. 2015 Sep 18;10(9):2126-34. doi: 
            10.1021/acschembio.5b00211. Epub 2015 Jul 20.
  PUBMED    26151333
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.5b00211"; journalName: "ACS Chem Biol"; date: 
            "2015-09-18- 18"; volume: "10"; issue: "9"; pages: "2126-34"
COMMENT     Sequence Label: pER-eZinCh-2
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(28..738,739..741,742..744))
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSHLCKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     419..438
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     678..699
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gggcccttac agctcgtcct tgtcgacctt gtacagctcg tccatgccga gagtgatccc
       61 ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgca
      121 caggtgggac tggtagctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat
      181 gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg
      241 gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg gccatgatat agacgttgtg
      301 gctgttgtag ttgtactcaa gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat
      361 gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt
      421 cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat
      481 ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcgggcga agcacatcag
      541 gccgtagccg aaggtggtca cgagggtggg ccagggcacg ggcagcttgc cggtggtgca
      601 gatgaacttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct
      661 gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca ccccggtgaa
      721 cagctcctcg cccttgctca ccatgggtac cgagccccgc ggacccccgg atccaccgct
      781 accaccggat ccaccgcttc caccggatcc accgctacca ccggatccac cgcttccacc
      841 ggatccgccg ctgccgccgg atccgccgga gccgccggat ccgccgctac cgccggatcc
      901 gccgcttccg ccggatccgc cacgaatgag ctcgccgcct ccggacttgt acagctcgtc
      961 catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt
     1021 ctcgttgggg tctt
//
