LOCUS       sequence-127773-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the S. Lycopersicum metallothionein II-like protein (MTB) 
            gene promoter as a level 0 GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pPMtb  (GB0080)
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             131..142
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ctccggccgc catggcggcc gcgggaattc gattgggcga tgagtggtct cgcattgatg
       61 attggtctga aacaagaatt gagaaatcaa atgtgatttt tttttttttt gattggtgta
      121 gaaatgagtg attgagggaa gaagcatgag tgtctattta tagtgaaacg aaggtacatg
      181 gatgatatta accgtacacg tgtcgtgatc gtatgggaat atgttatcct tgtaagtcgg
      241 tccatacatg atcctaattt catccacgta ttcgtcgttt tctataatca caattaccat
      301 tcgtacgtat gttctagaca tatttacatt ctctatcgac cgcgacttat ttatctatat
      361 cgaatgttta taacaaacta aataacttta tattttgaca cttttcaaga tttaaatgat
      421 tttatctttg attgtaaatt ttttatattt atttttattt attttgaatt aacagttatt
      481 gtgacctata gtacttttta tatagttgta taaattttat tttaaaaaat agaaaatttc
      541 gcgcgtatgt tttcggtcaa actttaatta tttgactctt gaaaaacgaa aagtgtcaca
      601 taaatcgaaa caaatgaagt atattgtgat cgagctgatg tagcatatga gatgataggt
      661 gcgtttgggt ggtggtcagg gatcggctaa aaaaataatt tagaattaaa tttttttaaa
      721 aagattgttg tttttcctaa aataaaaatg taattttaaa tttgaggaga gtttggaaaa
      781 atgttttcct taatttttta aaggaaatca tttttcttaa ttttgaggaa aatgagttga
      841 ttcgaaa
//
