LOCUS       sequence-127775-         650 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTET pduP1-18-mCherry-ssrA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Jakobson CM, Kim EY, Slininger MF, Chien A, Tullman-Ercek D
  TITLE     Localization of Proteins to the 1,2-Propanediol Utilization 
            Microcompartment by Non-native Signal Sequences Is Mediated by a 
            Common Hydrophobic Motif.
  JOURNAL   J Biol Chem. 2015 Aug 17. pii: jbc.M115.651919.
  PUBMED    26283792
REFERENCE   2  (bases 1 to 650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2015 Aug 17. pii: jbc.M115.651919."
COMMENT     Sequence Label: CMJ205
FEATURES             Location/Qualifiers
     source          1..650
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             177..188
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        201..256
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    202..220
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(348..367)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt cacggagccc tccatgtgca
       61 ccttgaagcg catgaactcc ttgatgatgg ccatgttatc ctcctcgccc ttgctcacta
      121 attgctcgct aagaatggtg cgaatcaggg tttcgagttc agaagtattc atgacctttc
      181 tcctctttaa gaattctctt ttctctatca ctgataggga gtggtaaaat aactctatca
      241 acgatagagt gtcaacaaaa attaggaatt aatgatgtct agattagata aaagtaaagt
      301 gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa
      361 actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa aaaataagcg
      421 ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc acttttgccc
      481 tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt ttagatgtgc
      541 tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta cagaaaaaca
      601 gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt
//
