LOCUS       sequence-127795-         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked bleMX marker, integration into S. cerevisiae XI-5 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2196
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    183..216
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt
       61 ggggactccc catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt
      121 cgaaagactg ggcctttcgc ccgggctaat tatggggtgt cgcccttatt cgactctata
      181 gtgaagttcc tattctctag aaagtatagg aacttctgaa gtggggattt aaatgcggcc
      241 gcgctgaggg tttaatgcgg agaagtcgtt gatagcattt ccgaaggctt ttccacattg
      301 actgcagtat ctaaaagagc tccagcagcg gtcttagtag ttcctgtgct tgccgctttg
      361 cagtatacag ggacattggg aactacggag gaaaaatcgg aatgctctga gctcttctca
      421 aaaccagacg aactcgtctt atttctcagt aaattaccca acatataaac tctccctctt
      481 caccttctct acaaggccac acacaagaca gttttaacag gcaaatggta ctggaggctc
      541 tcaccaatgg ggtttaagac gctccaaaca cacgctctcc tttttttctt tatatatcgg
      601 tagcaacgaa agctagtcgc agaacgttac acactaaagc agatcaatca atcaatcctg
      661 agaacctgct tcgttcaaac catccactag aagaaacacg cacgtgtacc caaaaataga
      721 gacaaacaaa cagacaaaaa taacaatgat tcctaccgac taaagtaaaa caacactcac
      781 gtaataaaaa atcctagata caactcactt gtattcctct cttctttgac catcactaaa
      841 gcttctctct tactatattc ctcaaataaa aaaaagttgc aatgattact taccaatgtg
      901 ccataaactc cgtgcaccaa ttaagtcctc agcgagctcg catggaatgc gtgcgatgag
      961 cgacctcatg ct
//
