LOCUS       sequence-127796-        1056 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked bleMX marker, integration into S. cerevisiae XI-5 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2196
FEATURES             Location/Qualifiers
     source          1..1056
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      100..289
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     protein_bind    337..370
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        425..768
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 acttatttaa taataaaaat cataaatcat aagaaattcg cttatttaga agtgtcaaca
       61 acgtatctac caacggaatg cgtgcgatcg cgtgcattca tccgctctaa ccgaaaagga
      121 aggagttaga caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt
      181 aagaacgtta tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg
      241 taacattata ctgaaaacct tgcttgagaa ggttttggga cgctcgaaga tcgcgtcagc
      301 tgaagcttcg tacgctgcag gtcgacaacc cttaatataa cttcgtataa tgtatgctat
      361 acgaagttat taggtctaga gatctgttta gcttgcctcg tccccgccgg gtcacccggc
      421 cagcgacatg gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc
      481 atgatgtgac tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc
      541 catacatttt gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg
      601 cgagcaggga aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta
      661 gagaaatata aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa
      721 aatcttgcta ggatacagtt ctcacatcac atccgaacat aaacaaccat ggccgaccaa
      781 gcgacgccca acctgccatc acgagatttc gattccacgg ccgccttcta tgaaaggttg
      841 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcaag
      901 ctggagttct tcgcccaccc cgggctcgat cccctcgcga gttggttcag ctgctgcctg
      961 aggctggacg acctcgcgga gttctaccgg cagtgcaaat ccgtcggcat ccaggaaacc
     1021 agcagcggct atccgcgcat ccatgccccc gaactg
//
