LOCUS       sequence-127801-         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked hphMX marker, integration into S. cerevisiae XII-5 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2337
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     68..85
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     69..85
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    555..588
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 ggcatcaaac taagcagaag gcccctgacg gatggccttt ttgcgtttct acaaactctt
       61 tctgtgttgt aaaacgacgg ccagtcttaa gctcgggccc cctgggcggt tctgataacg
      121 agtaatcgtt aatccgcaaa taacgtaaaa acccgcttcg gcgggttttt ttatgggggg
      181 agtttaggga aagagcattt gtcagaatat ttaagggcgc ctgtcacttt gcttgatata
      241 tgagaattat ttaaccttat aaatgagaaa aaagcaacgc actttaaata agatacgttg
      301 ctttttcgat tgatgaacac ctataattaa actattcatc tattatttat gattttttgt
      361 atatacaata tttctagttt gttaaagaga attaagaaaa taaatctcga aaataataaa
      421 gggaaaatca gtttttgata tcaaaattat acatgtcaac gataatacaa aatataatac
      481 aaactataag atgttatcag tatttattat gcatttagaa taaattttgt gtcgccctta
      541 ttcgactcac tatagaagtt cctattctct agaaagtata ggaacttcac ttcattttat
      601 ttaaatttgc ggccgcgctg aggtcttaat ctgcgatacc ttttgtgatg gctaaacaaa
      661 cagacatctt tttatatgtt tttacttctg tatatcgtga agtagtaagt gataagcgaa
      721 tttggctaag aacgttgtaa gtgaacaagg gacctctttt gcctttcaaa aaaggattaa
      781 atagagttaa tcattgagat ttagttttcg ttagattctg tatccctaaa taactccctt
      841 acccgacggg aaggcacaaa agacttgaat aatagcaaac ggccagtagc caagaccaaa
      901 taatactaga gttaactgat ggtcttaaac aggcattacg tggtgaac
//
