LOCUS       sequence-127809-         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked hphMX marker, integration into S. cerevisiae XII-4 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2198
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    186..219
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      complement(802..967)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     888..912
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 ggtggtccca cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag
       61 tgtggggact ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc
      121 agtcgaaaga ctgggccttt cgcccgggct aattatgggg tgtcgccctt attcgactct
      181 atagtgaagt tcctattctc tagaaagtat aggaacttct gaagtgggga tttaaatgcg
      241 gccgcgctga gggtttaatg tatccggctg ttccttcata gccctttcaa tgaacgttgc
      301 agccctttga agattggcca ttttgtcagg actcgagcct gacagttgga ccaacgcaac
      361 tttaattttt tgtgaaagaa tcttcgaagc actcatactg gcgatcttca cgccctcctg
      421 ctattacaaa agctgtgttt ttacaagaat caaattaagt tagcaagata ttatacaaca
      481 ttattgataa tttcaatatc gtgttcgtac ctgatgacgt atctgtgcat tgataaggcc
      541 cgcatggttt cagaaagcag agcggaacga ttccaaatta gtggccttgt gctttgcatg
      601 tcaattgtgt taccttcagc tcgtggattt gttttatcaa tacacagtct acagtcaaga
      661 attttttttt atcaaatttt gcgttcgagc gtataaaata gccgctgtag ctacttaagt
      721 tcctgttcag cgatagtttt tttccatcac acgtactatg gcaattaagt cctcagcgag
      781 ctcgcatgga atgcgtgcga tgagcgacct catgctatac ctgagaaagc aacctgacct
      841 acaggaaaga gttactcaag aataagaatt ttcgttttaa aacctaagag tcactttaaa
      901 atttgtatac acttattttt tttataactt atttaataat aaaaatcata aatcataaga
      961 aattcgctta tttagaagtg tcaaca
//
