LOCUS       sequence-127810-        1260 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway DONR vector with cassette for expression of 10 intronless S.
            cerevisiae tRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Cherry PD, White LK, York K, Hesselberth JR
  TITLE     Genetic bypass of essential RNA repair enzymes in budding yeast.
  JOURNAL   RNA. 2018 Mar;24(3):313-323. doi: 10.1261/rna.061788.117. Epub 2017 
            Dec 6.
  PUBMED    29212664
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.061788.117"; journalName: "RNA"; date: "2018-03"; 
            volume: "24"; issue: "3"; pages: "313-323"
COMMENT     Sequence Label: pDONR221-10X-tRNA
FEATURES             Location/Qualifiers
     source          1..1260
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    5..29
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      1161..1223
                     /label=RPR1 terminator
                     /note="transcription terminator for the S. cerevisiae RNase
                     P RNA gene"
     protein_bind    complement(1232..1256)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggggacaagt ttgtacaaaa aagcaggctg agctcccatc ttggaaggac cggataatta
       61 tttgaaatct ctttttcaat tgtatatgtg ttatgtagta tactctttct tcaacaatta
      121 aatactctcg gtagccaagt tggtttaagg cgcaagactg taatttatca ctacgaaatc
      181 ttgagatcgg gcgttcgact cgcccccggg agaacaaata gtagcggact tagctcagtt
      241 gggagagcgc cagactgaag atctggaggt cctgtgttcg atccacagag ttcgcacttt
      301 gtttcttaca aatagtaggt tgtttggccg agcggtctaa ggcgcctgat tcaagctcag
      361 gtatcgtaag atgcaagagt tcgaatctct tagcaaccac tttgtttctt acaaatagta
      421 tccttgttag ctcagttggt agagcgttcg gcttttaacc gaaatgtcag gggttcgagc
      481 cccctatgag gagctttgtt tcttacaaat agtagtccca gtggccgagt ggttaaggcg
      541 atgccctgct aaggcattgg gttttacctg cgcaggttcg aatcctgtct gtgacgcttt
      601 gtttcttaca aatagtagct cgtgtagctc agtggttaga gcttcgtgct tataacgcga
      661 ccgtcgtggg ttcaaacccc acctcgagca ctttgtttct tacaaatagt agaagcggtg
      721 gctcaatggt agagctttcg actccaaatc gaagggttgc aggttcaatt cctgtccgtt
      781 tcactttgtc tctcggtagc caagttggtt taaggcgcaa gactgtaaat cttgagatcg
      841 ggcgttcgac tcgcccccgg gagactttgt gggcgtgtgg tctagtggta tgattctcgc
      901 tttgggtgcg agaggccctg ggttcaattc ccagctcgcc ccctttgttt cttacaaata
      961 gtaggcacta tggccgagtg gttaaggcga gagactcgaa atctcttggg ctctgcccgc
     1021 gctggttcaa atcctgctgg tgtcgctttg tttcttacaa atagtaggga gtttggccga
     1081 gtggtttaag gcgtcagatt taggctctga tatcttcgga tgcaagggtt cgaatccctt
     1141 agctctcact ttgtttcttc ccatatccaa cttccaattt aatctttctt ttttaatttt
     1201 cacttatttg cgatacagaa agaaaatcga tacccagctt tcttgtacaa agtggtcccc
//
