LOCUS       sequence-127814-         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked natMX marker, integration into S. cerevisiae X-2 
            chromosomal location, USER site for cloning, amp resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2193
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    183..216
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt
       61 ggggactccc catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt
      121 cgaaagactg ggcctttcgc ccgggctaat tatggggtgt cgcccttatt cgactctata
      181 gtgaagttcc tattctctag aaagtatagg aacttctgaa gtggggattt aaatgcggcc
      241 gcgctgaggg tttaatcgtc tatgaggaga ctgttagttg gatatcagta atgagacgaa
      301 aaagctcgaa atgaatggat atattctttt tgctactggc aactgttgaa tatttaatgt
      361 taaaacaaac taactgaggt atattcgtat ctgtatgtac acatatacta tatacaggaa
      421 aagataagca agagagagga tatcaactac gagagcgatc gattatatat caaaagctgt
      481 ccgctttgcc acccataatc ggcgcttagt ttcggagttc aatcataatt ctaccacctt
      541 acactcaact tactctttaa ctcctatagt ataatatcgc cactgacccc atattaaaaa
      601 atttttttgc tcgatcttct atcctcttta ggttaattgt cgctgttatt gtctagattt
      661 tttctcggag atggcgcatc tatttgccgt caaaagatcc tctcatacca tattaagtaa
      721 attgcctcca tttctttttc ctcgggc
//
