LOCUS       sequence-127821-         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked kanMX marker, integration into S. cerevisiae X-3 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2223
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..73
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     57..73
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    543..576
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 agcagaaggc ccctgacgga tggccttttt gcgtttctac aaactctttc tgtgttgtaa
       61 aacgacggcc agtcttaagc tcgggccccc tgggcggttc tgataacgag taatcgttaa
      121 tccgcaaata acgtaaaaac ccgcttcggc gggttttttt atggggggag tttagggaaa
      181 gagcatttgt cagaatattt aagggcgcct gtcactttgc ttgatatatg agaattattt
      241 aaccttataa atgagaaaaa agcaacgcac tttaaataag atacgttgct ttttcgattg
      301 atgaacacct ataattaaac tattcatcta ttatttatga ttttttgtat atacaatatt
      361 tctagtttgt taaagagaat taagaaaata aatctcgaaa ataataaagg gaaaatcagt
      421 ttttgatatc aaaattatac atgtcaacga taatacaaaa tataatacaa actataagat
      481 gttatcagta tttattatgc atttagaata aattttgtgt cgcccttatt cgactcacta
      541 tagaagttcc tattctctag aaagtatagg aacttcactt cattttattt aaatttgcgg
      601 ccgcgctgag gtcttaatga ggtggttatt gatcaccgga aagtttggca ttaaaaacaa
      661 acgacagcac aaaggaactt tcaccctaca gcaccttctg tgtgcatgat tacatgtacc
      721 cgagtagcga aaaaagccag aaaatgtgca agaataaagg tatctcaatg aacgagctcg
      781 agcgtca
//
