LOCUS       sequence-127822-        1019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses myc::TEV::3Xflag::eGFP::3Xflag::TEV::myc in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Paix A, Folkmann A, Rasoloson D, Seydoux G
  TITLE     High Efficiency, Homology-Directed Genome Editing in Caenorhabditis 
            elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes.
  JOURNAL   Genetics. 2015 Sep;201(1):47-54. doi: 10.1534/genetics.115.179382. 
            Epub 2015 Jul 17.
  PUBMED    26187122
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.115.179382"; journalName: "Genetics"; date: 
            "2015-09"; volume: "201"; issue: "1"; pages: "47-54"
COMMENT     Sequence Label: AP625-1
FEATURES             Location/Qualifiers
     source          1..1019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..50
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             51..71
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             72..137
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             join(138..140,141..851)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(168..196)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     791..811
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             852..917
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             918..938
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             939..968
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gagctcggta cccggggatc gagcaaaagc tcatctccga ggaggacctc gaaaatttat
       61 attttcaggg tgactacaag gaccacgacg gagactacaa ggaccacgac atcgactaca
      121 aggacgacga cgacaaggtg agtaaaggag aagaactttt cactggagtt gtcccaattc
      181 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag
      241 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg
      301 ttccatggcc aacacttgtc actactctga cctacggcgt gcagtgcttc agccggtacc
      361 ccgaccacat gaagcagcat gactttttca agagtgccat gcccgaaggt tatgtacagg
      421 aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg
      481 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa
      541 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag
      601 acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa
      661 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt
      721 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga
      781 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacactc ggcatggacg
      841 agctgtacaa ggactacaaa gaccatgacg gtgattataa agatcatgat atcgattaca
      901 aggatgacga tgacaaggag aacctctact tccaaggaga acaaaaactg atatcagaag
      961 aggatctgga tcctctagag tcgacctgca ggcatgcaag cttggcgtaa tcatggtca
//
