LOCUS       sequence-127825-        1036 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses myc::TEV::3Xflag::eGFP::3Xflag::TEV::myc in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Paix A, Folkmann A, Rasoloson D, Seydoux G
  TITLE     High Efficiency, Homology-Directed Genome Editing in Caenorhabditis 
            elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes.
  JOURNAL   Genetics. 2015 Sep;201(1):47-54. doi: 10.1534/genetics.115.179382. 
            Epub 2015 Jul 17.
  PUBMED    26187122
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.115.179382"; journalName: "Genetics"; date: 
            "2015-09"; volume: "201"; issue: "1"; pages: "47-54"
COMMENT     Sequence Label: AP625-1
FEATURES             Location/Qualifiers
     source          1..1036
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(48..77)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(78..98)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(99..164)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(join(165..875,876..878))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(205..225)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     820..848
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     CDS             complement(879..944)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(945..965)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(966..995)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 catgattacg ccaagcttgc atgcctgcag gtcgactcta gaggatccag atcctcttct
       61 gatatcagtt tttgttctcc ttggaagtag aggttctcct tgtcatcgtc atccttgtaa
      121 tcgatatcat gatctttata atcaccgtca tggtctttgt agtccttgta cagctcgtcc
      181 atgccgagtg taatcccagc agctgttaca aactcaagaa ggaccatgtg gtctctcttt
      241 tcgttgggat ctttcgaaag ggcagattgt gtggacaggt aatggttgtc tggtaaaagg
      301 acagggccat cgccaattgg agtattttgt tgataatggt ctgctagttg aacgcttcca
      361 tcttcaatgt tgtgtctaat tttgaagtta actttgattc cattcttttg tttgtctgcc
      421 atgatgtata cattgtgtga gttatagttg tattccaatt tgtgtccaag aatgtttcca
      481 tcttctttaa aatcaatacc ttttaactcg attctattaa caagggtatc accttcaaac
      541 ttgacttcag cacgtgtctt gtagttcccg tcatctttga aaaatatagt tctttcctgt
      601 acataacctt cgggcatggc actcttgaaa aagtcatgct gcttcatgtg gtcggggtac
      661 cggctgaagc actgcacgcc gtaggtcaga gtagtgacaa gtgttggcca tggaacaggt
      721 agttttccag tagtgcaaat aaatttaagg gtaagttttc cgtatgttgc atcaccttca
      781 ccctctccac tgacagaaaa tttgtgccca ttaacatcac catctaattc aacaagaatt
      841 gggacaactc cagtgaaaag ttcttctcct ttactcacct tgtcgtcgtc gtccttgtag
      901 tcgatgtcgt ggtccttgta gtctccgtcg tggtccttgt agtcaccctg aaaatataaa
      961 ttttcgaggt cctcctcgga gatgagcttt tgctcgatcc ccgggtaccg agctcgaatt
     1021 cactggccgt cgtttt
//
