LOCUS       sequence-127826-         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked kanMX marker, integration into S. cerevisiae X-3 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2223
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    186..219
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 ggtggtccca cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag
       61 tgtggggact ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc
      121 agtcgaaaga ctgggccttt cgcccgggct aattatgggg tgtcgccctt attcgactct
      181 atagtgaagt tcctattctc tagaaagtat aggaacttct gaagtgggga tttaaatgcg
      241 gccgcgctga gggtttaatc gagatctttg tgttcggtta cccggctcag atcctaactt
      301 cttcttttgg tatgtttatt cgtataagtt actgttgtcc acaggcaata ctctgcagaa
      361 aattaaaacg gcattaatgc taggacaacc agaattgtta ctactgtatg tgcgatagtt
      421 gataactgca acattatgcc cggtatattc tcaaaaaacc ctattactgc atacgaagaa
      481 atcgcaagag aaatctttcg gtttggaaaa gctcactgtg aggttccttg gagccaatag
      541 taatacagca caatccaagg aaaaatctgg cctatatgca aggaaggaga gatagtcaaa
      601 agcattcttt cccctagaag ttggtgcata tatggcatcg ttaaaacata ttacccccaa
      661 aatttcttct ctaaacgatg tgcttggcct ttgttttggt ttttgatgtc ggtcgtttga
      721 ggccccttgc ggaaaatcga gatcgccgaa atgtgcactc gaa
//
