LOCUS       sequence-127831-         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked hphMX marker, integration into S. cerevisiae X-4 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2194
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     72..89
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     73..89
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    559..592
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 gccaggcatc aaactaagca gaaggcccct gacggatggc ctttttgcgt ttctacaaac
       61 tctttctgtg ttgtaaaacg acggccagtc ttaagctcgg gccccctggg cggttctgat
      121 aacgagtaat cgttaatccg caaataacgt aaaaacccgc ttcggcgggt ttttttatgg
      181 ggggagttta gggaaagagc atttgtcaga atatttaagg gcgcctgtca ctttgcttga
      241 tatatgagaa ttatttaacc ttataaatga gaaaaaagca acgcacttta aataagatac
      301 gttgcttttt cgattgatga acacctataa ttaaactatt catctattat ttatgatttt
      361 ttgtatatac aatatttcta gtttgttaaa gagaattaag aaaataaatc tcgaaaataa
      421 taaagggaaa atcagttttt gatatcaaaa ttatacatgt caacgataat acaaaatata
      481 atacaaacta taagatgtta tcagtattta ttatgcattt agaataaatt ttgtgtcgcc
      541 cttattcgac tcactataga agttcctatt ctctagaaag tataggaact tcacttcatt
      601 ttatttaaat ttgcggccgc gctgaggtct taatctggtg aggatttacg gtatgatcat
      661 gctggtaagc ttcgttactc atgctttggt tggcttcttg aaagtgtcta gttcacttta
      721 tgcccggtta taaacataaa ctttcacagg gttctctttg atcacctctt tgtttaatct
      781 tatgaatcca agggatttta tcggtcaaag gggaaatcaa tgcgaaagac agtaatgatg
      841 agagaaaaac tctccgtaac caccaagttt ggttcagcgc gacgagattt ttatcgatta
      901 tcgagaaaaa tacctgtata tctacatttc tatgtca
//
