LOCUS       sequence-127833-         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked hphMX marker, integration into S. cerevisiae X-4 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2194
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    183..216
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     903..927
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt
       61 ggggactccc catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt
      121 cgaaagactg ggcctttcgc ccgggctaat tatggggtgt cgcccttatt cgactctata
      181 gtgaagttcc tattctctag aaagtatagg aacttctgaa gtggggattt aaatgcggcc
      241 gcgctgaggg tttaatccca aagctaagag tcccatttta ttcttctata tgtatatttt
      301 cgatactcta aaccacccta caatgtagcc ctatactaaa tctgctcaat tttcagcttc
      361 tacaagtgac tcgaggccac gtggaaagat ccaactactc cagcacaacg attcaatata
      421 atcgattgct ccactcataa gaggcaagaa caagcttcaa cttttggtaa gccgccgttt
      481 ataaacaggg aagatgtcct ttgtcaaggg aggcacagag catggccaat ttggcaaatt
      541 gcaggttttt ctgagtgaaa aatgaaaaag cattgtagta gagtcggctc actgaaaaac
      601 cggggaggac gaaaaggttt ccagccacag ttgtagtcac gtgcgcgcca tgctgactaa
      661 tggcagccgt cgttgggcag aagagaatta gtatggtaca ggatacgcta attgcgctcc
      721 aactaccaag gttgttgagg gaacactggg gcaataggct gtcgccattc aagagcagat
      781 taagtcctca gcgagctcgc atggaatgcg tgcgatgagc gacctcatgc tatacctgag
      841 aaagcaacct gacctacagg aaagagttac tcaagaataa gaattttcgt tttaaaacct
      901 aagagtcact ttaaaatttg tatacactta ttttttttat aacttattta ataataaaaa
      961 tc
//
