LOCUS       sequence-127834-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  co-expression of Cas9 and crRNA 589 target sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Paix A, Folkmann A, Rasoloson D, Seydoux G
  TITLE     High Efficiency, Homology-Directed Genome Editing in Caenorhabditis 
            elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes.
  JOURNAL   Genetics. 2015 Sep;201(1):47-54. doi: 10.1534/genetics.115.179382. 
            Epub 2015 Jul 17.
  PUBMED    26187122
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.115.179382"; journalName: "Genetics"; date: 
            "2015-09"; volume: "201"; issue: "1"; pages: "47-54"
COMMENT     Sequence Label: AP568-2
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(23..346)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     CDS             complement(361..387)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(388..408)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttcaactata ataaagttgt gagacttttt tcttggcggc acaataaagt ttcctctttt
       61 tgttgggtca ctcaacgaac acaatacaaa tatttgagcg ctcgcatttt tctcaacctt
      121 tcgggtttaa agcacttcaa aatggaattg aagagagatt gtgaaagtac atcagacaga
      181 atcacatttt gatagcattc acttcactca gatgcaaact tttcagtgac tataaaaagt
      241 cataaaataa taacattaaa aaagcaaata aattagcgag agaatttttt gacaaaaaga
      301 aagaagagtg atagagaaga agggaatgct tgaaaggatt ttgcatttat cactagttta
      361 ggcgtagtct gggacgtcgt atgggtagac cttacgcttc ttctttggtg gtccggatcc
      421 tccgtctcct ccgagttggg agaggtcgat acgggtctcg tagagtccgg tgatggattg
      481 gtggatgagg gtggcgtcga ggacctcctt ggtggaggtg taacgcttac ggtcgatggt
      541 ggtgtcgaag tacttgaagg cggctggggc tccgaggttg gtgagggtga agaggtggat
      601 gatgttctcg gcttgctcac ggattggctt gtcacggtgc ttgttgtagg cggagaggac
      661 cttgtcgagg ttggcgtcgg cgaggatgac acgcttggag aactcggaga tttgctcgat
      721 gatctcgtcg aggtagtgct tgtgttgctc gacgaagagt tgcttttgct c
//
