LOCUS       sequence-127840-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked KlURA3 marker, integration into S. cerevisiae XI-1 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2374
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      95..284
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     protein_bind    332..365
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tttaataata aaaatcataa atcataagaa attcgcttat ttagaagtgt caacaacgta
       61 tctaccaacg gaatgcgtgc gatcgcgtgc attcatccgc tctaaccgaa aaggaaggag
      121 ttagacaacc tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa
      181 cgttatttat atttcaaatt tttctttttt ttctgtacag acgcgtgtac gcatgtaaca
      241 ttatactgaa aaccttgctt gagaaggttt tgggacgctc gaagatcgcg tcagctgaag
      301 cttcgtacgc tgcaggtcga caacccttaa tataacttcg tataatgtat gctatacgaa
      361 gttattaggt ctagagatcc caatacaaca gatcacgtga tctttttgta agatgaagtt
      421 gaagtagtgt tgcaccgtgc caatgcaggt ggctattaga ttaaatatgt gatttgttct
      481 attaagtttc ctgtataatt aatggggagc gctgattctc ttttggtacg cttcccatcc
      541 agcatttctg tatctttcac cttcaacctt aggatctcta cccttggcga aaagtcctct
      601 gccaacaatg atgatatctg atccaccact tacaacttcg tcgacggttc tgtactgctg
      661 acccaatgca tcgcctttgt cgtctaaacc tacacctggg gtcatgatta gccaatcaaa
      721 cccttcttct cttcctccca tatcgttctg agcaatgaac ccaataacga aatctttatc
      781 actctttgca atatcaacgg tacccttagt atattcaccg tgtgctagag aacccttgga
      841 agataattca gcaagcatca ataatcccct tggttctttg gtgacctctt gcgcaccttg
      901 tttcaagcca gcaacaatac cagcaccagt aaccccgtgg gcgttggtga tatcagacca
      961 ttctgcgata cggtaaacgc ccgatgtata ttgtaatttg actgtgttac cg
//
