LOCUS       sequence-127847-         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked KlURA3 marker, integration into S. cerevisiae XI-1 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2374
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    182..215
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     841..865
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg
       61 gggactcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc
      121 gaaagactgg gcctttcgcc cgggctaatt atggggtgtc gcccttattc gactctatag
      181 tgaagttcct attctctaga aagtatagga acttctgaag tggggattta aatgcggccg
      241 cgctgagggt ttaatggaat agtgacgttg tgatgcggtg agttcggcgg ttaggggaat
      301 ggtatatgat aaaaaacgga aacgtgcttc tttaatttaa ttgtttaata ttgttgcaga
      361 tatataaaaa gggggaaaga accgaagatg taattatttt tttatcgcct caacctaaag
      421 caagcaataa ggtataaaga tcaggacgtc tcgagcgctg atatctaaat ttgaagccac
      481 gcaagtaact acgtaggtca gaggggacaa ggaataacac ttgacatttt tcttttttct
      541 ttttttttct tttttttttt tttgttaatc ttggcttctg taccgtagcc tcctgtatac
      601 ggttttagat gcagtatgtt tatcatcgcc gagaaatttg gggggtgcaa aggaataaaa
      661 tccgtgctga aatccgtgca ccgcatcaaa ctttctcgga ggattctttg cagccgatta
      721 agtcctcagc gagctcgcat ggaatgcgtg cgatgagcga cctcatgcta tacctgagaa
      781 agcaacctga cctacaggaa agagttactc aagaataaga attttcgttt taaaacctaa
      841 gagtcacttt aaaatttgta tacacttatt ttttttataa cttatttaat aataaaaatc
      901 ataaat
//
