LOCUS       sequence-127849-         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the CaMV dual 35S Promoter as a level 0 GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: p2x35S  (GB0222)
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(129..473)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(165..185)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 ctccggccgc catggcggcc gcgggaattc gattgggcga tgagtggtct cgcatttcga
       61 ctagaatagt aaattgtaat gttgtttgtt gtttgttttg ttgtggtaat tgttgtaaaa
      121 ataccggagt cctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag
      181 gatagtggga ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct
      241 ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat
      301 ctttgggacc actgtcggca ggggcatctt gaacgatagc ctttccttta tcgcaatgat
      361 ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg
      421 ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcattgcgaa
      481 ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc
      541 tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca
      601 tctttgggac cactgtcggc aggggcatct tgaacgatag cctttccttt atcgcaatga
      661 tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct
      721 gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaag
//
