LOCUS       sequence-127850-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked KlLEU2 marker, integration into S. cerevisiae XI-2 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2190
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      81..270
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     protein_bind    318..351
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tcataaatca taagaaattc gcttatttag aagtgtcaac aacgtatcta ccaacggaat
       61 gcgtgcgatc gcgtgcattc atccgctcta accgaaaagg aaggagttag acaacctgaa
      121 gtctaggtcc ctatttattt ttttatagtt atgttagtat taagaacgtt atttatattt
      181 caaatttttc ttttttttct gtacagacgc gtgtacgcat gtaacattat actgaaaacc
      241 ttgcttgaga aggttttggg acgctcgaag atcgcgtcag ctgaagcttc gtacgctgca
      301 ggtcgacaac ccttaatata acttcgtata atgtatgcta tacgaagtta ttaggtctag
      361 agatccgcag gctaaccgga acctgtatta tttagtttat gctacgttaa ataaagacct
      421 ttcgttcaca taactgaatg tgtaatggcc ttgagatttc aagcatacca agttggtgga
      481 gacggggtcg ttacaaaaga ctctttaagc caagatttcc ttgacagcct tggcgatagc
      541 atcgccaacc tcagtggtag agttagaacc accaaggtca ccggttctga cacctgcatc
      601 caagacattt ctaacagctt cttcaagagc cctaccttct tcaaccaaat ccaaggataa
      661 cttcaacatc atagctgcag ataagatggt agcaattggg ttaaccttgt ttgctggtaa
      721 atctggggca gaaccatgac atggttcgta caaaccgaat gccttgttag tgtcaggtag
      781 ggaagctaga gatgcagaag gtaataaacc caaagaacct ggaataacag aggctt
//
