LOCUS       sequence-127858-         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  co-expression of Cas9 and crRNA 589 target sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Paix A, Folkmann A, Rasoloson D, Seydoux G
  TITLE     High Efficiency, Homology-Directed Genome Editing in Caenorhabditis 
            elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes.
  JOURNAL   Genetics. 2015 Sep;201(1):47-54. doi: 10.1534/genetics.115.179382. 
            Epub 2015 Jul 17.
  PUBMED    26187122
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.115.179382"; journalName: "Genetics"; date: 
            "2015-09"; volume: "201"; issue: "1"; pages: "47-54"
COMMENT     Sequence Label: AP568-3
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     11..29
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(67..89)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_RNA        complement(361..436)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(457..806)
                     /label=CeU6 promoter
                     /note="promoter for the C. elegans R07E5.16 U6 snRNA gene 
                     (Dickinson et al., 2013)"
     primer_bind     812..834
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     826..843
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     827..843
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    851..871
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
       61 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
      121 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
      181 taactatgcg gcatcagagc agattgcact gagagtgcac catatgcggt gtgaaatacc
      241 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
      301 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagaaat ttcacaaaaa
      361 gcaccgactc ggtgccactt tttcaagttg ataacggact agccttattt taacttgcta
      421 tttctagctc taaaacctcg tggtgcctat ggtagcaaga catctcgcaa taggaggtgt
      481 ttatataggg gcaacacaag aggcggcaga catctcgtga gtttgacaga gtagtataaa
      541 gagtgtgtgt gagagggtgt aggcatttca taacatattt tttgtggaat aattttggaa
      601 aaaaggtgtt tttttaataa tagcttttat aagagtttct caaaaattgc atgctttttt
      661 tgaaaaggtt gacaaattat agtagtacta aagttgctat tttcaggtct cagcagaaaa
      721 ttcacaacta aaaataaaat ttgcaaaaca gagtgtgcgc ctttaaggtg tacagttttt
      781 tattccttta ttgctagttt tttttggttt tcccagtcac gacgttgtaa aacgacggcc
      841 agtgaattat caactatgta taataaagtt gtgagacttt ttt
//
