LOCUS       sequence-127861-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked KlLEU2 marker, integration into S. cerevisiae XI-2 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2190
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     75..92
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     76..92
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    562..595
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 actgccaggc atcaaactaa gcagaaggcc cctgacggat ggcctttttg cgtttctaca
       61 aactctttct gtgttgtaaa acgacggcca gtcttaagct cgggccccct gggcggttct
      121 gataacgagt aatcgttaat ccgcaaataa cgtaaaaacc cgcttcggcg ggttttttta
      181 tggggggagt ttagggaaag agcatttgtc agaatattta agggcgcctg tcactttgct
      241 tgatatatga gaattattta accttataaa tgagaaaaaa gcaacgcact ttaaataaga
      301 tacgttgctt tttcgattga tgaacaccta taattaaact attcatctat tatttatgat
      361 tttttgtata tacaatattt ctagtttgtt aaagagaatt aagaaaataa atctcgaaaa
      421 taataaaggg aaaatcagtt tttgatatca aaattataca tgtcaacgat aatacaaaat
      481 ataatacaaa ctataagatg ttatcagtat ttattatgca tttagaataa attttgtgtc
      541 gcccttattc gactcactat agaagttcct attctctaga aagtatagga acttcacttc
      601 attttattta aatttgcggc cgcgctgagg tcttaatgtg ggaagattcc gctctaccag
      661 ggaaaaacaa aattatatag aaaagaaggt aagtaataag taatacgaaa aaacccaaaa
      721 aaaaaattca aataccccaa ccctgtgccc tttctatttt tatggttgaa aaggttacag
      781 aggatcctct ggagcggtga caacagttga cgcaatcatg gtccctggcg gtccctaagg
      841 atatctcgga ggaacc
//
