LOCUS       sequence-127862-         575 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively expresses yellow fluorescent protein , EYFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Davidsohn N, Beal J, Kiani S, Adler A, Yaman F, Li Y, Xie Z, Weiss R
  TITLE     Accurate Predictions of Genetic Circuit Behavior from Part 
            Characterization and Modular Composition.
  JOURNAL   ACS Synth Biol. 2014 Nov 17.
  PUBMED    25369267
REFERENCE   2  (bases 1 to 575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Nov 17."
COMMENT     Sequence Label: pExp_CAG:EYFP
FEATURES             Location/Qualifiers
     source          1..575
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..83
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     75..92
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     76..92
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..118
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        99..117
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        227..530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 aatttctcga gttaattaaa tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
       61 cccagtcacg acgttgtaaa acgacggcca gtgaattgta atacgactca ctatagggcg
      121 aattggagct aggcctacgt agcgcgcgag ctccaccgcg gtggactagt tattaatagt
      181 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      241 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      301 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      361 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
      421 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
      481 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga
      541 gccccacgtt ctgcttcact ctccccatct ccccc
//
