LOCUS       sequence-127866-         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Cai Y, Jin J, Tomomori-Sato C, Sato S, Sorokina I, Parmely TJ, 
            Conaway RC, Conaway JW
  TITLE     Identification of new subunits of the multiprotein mammalian 
            TRRAP/TIP60-containing histone acetyltransferase complex.
  JOURNAL   J Biol Chem. 2003 Oct 31. 278(44):42733-6.
  PUBMED    12963728
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2003 Oct 31. 278(44):42733-6."
COMMENT     Sequence Label: FLAG-MRGBP
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        32..50
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             76..99
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(770..787)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
       61 ctggctagca tgccagacta caaggacgat gatgacaagc tcgagatggg agaggccgag
      121 gtgggcggcg ggggcgccgc aggcgacaag ggcccggggg aggcggccac cagcccggcg
      181 gaggagacag tggtgtggag ccccgaggtg gaggtgtgcc tcttccacgc catgctgggc
      241 cacaagcccg tcggtgtgaa ccgacacttc cacatgattt gtattcggga caagttcagc
      301 cagaacatcg ggcggcaggt cccatccaag gtcatctggg accatctgag caccatgtac
      361 gacatgcagg cgctgcatga gtctgagatt cttccattcc cgaatccaga gaggaacttc
      421 gtccttccag aagagatcat tcaggaggtc cgagaaggaa aagtgatgat agaagaggag
      481 atgaaagagg agatgaagga agacgtggac ccccacaatg gggctgacga tgttttttca
      541 tcttcaggga gtttggggaa agcatcagaa aaatccagca aagacaaaga gaagaactcc
      601 tcagacttgg ggtgcaaaga aggcgcagac aagcggaagc gcagccgggt caccgacaaa
      661 gtcctgaccg caaacagcaa cccttccagt cccagtgctg ccaagcggcg ccgcacgtag
      721 ggatccggta ccgtcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg
      781 ccttctagtt gccagccatc tgttgtttgc cc
//
