LOCUS       sequence-127875-        1069 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Ollas::mCherry::linker::H2B::V5 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Paix A, Folkmann A, Rasoloson D, Seydoux G
  TITLE     High Efficiency, Homology-Directed Genome Editing in Caenorhabditis 
            elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes.
  JOURNAL   Genetics. 2015 Sep;201(1):47-54. doi: 10.1534/genetics.115.179382. 
            Epub 2015 Jul 17.
  PUBMED    26187122
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.115.179382"; journalName: "Genetics"; date: 
            "2015-09"; volume: "201"; issue: "1"; pages: "47-54"
COMMENT     Sequence Label: AP582-2
FEATURES             Location/Qualifiers
     source          1..1069
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             23..64
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
ORIGIN
        1 tcgagctcgg tacccgggga tctccggatt cgccaacgag ctcggaccac gtctcatggg
       61 aaaggtctca aagggtgaag aagataacat ggcaattatt aaagagttta tgcgtttcaa
      121 ggtgcatatg gagggatctg tcaatgggca tgagtttgaa attgaaggtg aaggagaagg
      181 ccgaccatat gagggaacac aaaccgcaaa actaaaggta actaaaggcg gaccattacc
      241 attcgcctgg gacatcctct ctccacagtt catgtatgga agtaaagctt atgttaaaca
      301 tccggcagat ataccagatt atttgaaact ttcattcccg gagggtttta agtgggaacg
      361 cgtaatgaat tttgaagacg gaggagttgt tacagtgacg caagactcca gcctccaaga
      421 tggagaattt atttataaag tcaaacttcg aggaacgaat ttcccctcgg atggacctgt
      481 tatgcagaag aagactatgg gatgggaagc ttcaagtgaa agaatgtacc ctgaagacgg
      541 tgctcttaag ggagagatta aacaacgtct taaattgaaa gatggaggac attacgatgc
      601 tgaggtgaag acaacttaca aagccaaaaa accagttcag ctgccaggag cgtacaatgt
      661 taatattaaa ctggatatca cctcccacaa cgaggattac actatcgttg agcaatatga
      721 aagagctgaa gggcggcact cgacaggtgg catggatgaa ttgtataagt gcccggggga
      781 tcggtggagc tccaccggtg gcggccgctc tagaactagt ccaccaaagc catctgccaa
      841 gggagccaag aaggccgcca agaccgtcgt tgccaagcca aaggacggaa agaagagacg
      901 tcatgcccgc aaggaatcgt actccgtcta catctaccgt gttctcaagc aagttcaccc
      961 agacaccgga gtctcctcca aggccatgtc tatcatgaac tccttcgtca acgatgtatt
     1021 cgaacgcatc gcttcggaag cttcccgtct tgctcattac aacaaacgc
//
