LOCUS       sequence-127877-         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked CaLYS5 marker, integration into S. cerevisiae XI-5 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2229
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      87..276
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     protein_bind    324..357
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 taaaaatcat aaatcataag aaattcgctt atttagaagt gtcaacaacg tatctaccaa
       61 cggaatgcgt gcgatcgcgt gcattcatcc gctctaaccg aaaaggaagg agttagacaa
      121 cctgaagtct aggtccctat ttattttttt atagttatgt tagtattaag aacgttattt
      181 atatttcaaa tttttctttt ttttctgtac agacgcgtgt acgcatgtaa cattatactg
      241 aaaaccttgc ttgagaaggt tttgggacgc tcgaagatcg cgtcagctga agcttcgtac
      301 gctgcaggtc gacaaccctt aatataactt cgtataatgt atgctatacg aagttattag
      361 gtctagagat ctgtttagct tgcctcgtcc ccgccgggtc acccggccag cgacatggag
      421 gcccagaata ccctccttga cagtcttgac gtgcgcagct caggggcatg atgtgactgt
      481 cgcccgtaca tttagcccat acatccccat gtataatcat ttgcatccat acattttgat
      541 ggccgcacgg cgcgaagcaa aaattacggc tcctcgctgc agacctgcga gcagggaaac
      601 gctcccctca cagacgcgtt gaattgtccc cacgccgcgc ccctgtagag aaatataaaa
      661 ggttaggatt tgccactgag gttcttcttt catatacttc cttttaaaat cttgctagga
      721 tacagttctc a
//
