LOCUS       sequence-127880-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked CaLYS5 marker, integration into S. cerevisiae XI-5 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2229
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..66
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     50..66
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    536..569
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 ggcccctgac ggatggcctt tttgcgtttc tacaaactct ttctgtgttg taaaacgacg
       61 gccagtctta agctcgggcc ccctgggcgg ttctgataac gagtaatcgt taatccgcaa
      121 ataacgtaaa aacccgcttc ggcgggtttt tttatggggg gagtttaggg aaagagcatt
      181 tgtcagaata tttaagggcg cctgtcactt tgcttgatat atgagaatta tttaacctta
      241 taaatgagaa aaaagcaacg cactttaaat aagatacgtt gctttttcga ttgatgaaca
      301 cctataatta aactattcat ctattattta tgattttttg tatatacaat atttctagtt
      361 tgttaaagag aattaagaaa ataaatctcg aaaataataa agggaaaatc agtttttgat
      421 atcaaaatta tacatgtcaa cgataataca aaatataata caaactataa gatgttatca
      481 gtatttatta tgcatttaga ataaattttg tgtcgccctt attcgactca ctatagaagt
      541 tcctattctc tagaaagtat aggaacttca cttcatttta tttaaatttg cggccgcgct
      601 gaggtcttaa tgatcataga tccggcactt agagaaagac catagttgac caacttagat
      661 tggctattgc gcttgctatc gaaggcaacc gataacaacg gcacacacag ggccccaagt
      721 atgcacagca ccgacgatat aagagaaaac aacaaccacc tgggaatttt ttccattttc
      781 ttgcgtttct tcactgtg
//
