LOCUS       sequence-127882-         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked CaLYS5 marker, integration into S. cerevisiae XI-5 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2229
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    139..172
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 gcgccgatgg tagtgtgggg actccccatg cgagagtagg gaactgccag gcatcaaata
       61 aaacgaaagg ctcagtcgaa agactgggcc tttcgcccgg gctaattatg gggtgtcgcc
      121 cttattcgac tctatagtga agttcctatt ctctagaaag tataggaact tctgaagtgg
      181 ggatttaaat gcggccgcgc tgagggttta atgcggagaa gtcgttgata gcatttccga
      241 aggcttttcc acattgactg cagtatctaa aagagctcca gcagcggtct tagtagttcc
      301 tgtgcttgcc gctttgcagt atacagggac attgggaact acggaggaaa aatcggaatg
      361 ctctgagctc ttctcaaaac cagacgaact cgtcttattt ctcagtaaat tacccaacat
      421 ataaactctc cctcttcacc ttctctacaa ggccacacac aagacagttt taacaggcaa
      481 atggtactgg aggctctcac caatggggtt taagacgctc caaacacacg ctctcctttt
      541 tttctttata tatcggtagc aacgaaagct agtcgcagaa cgttacacac taaagcagat
      601 caatcaatca atcctgagaa cctgcttcgt tcaaaccatc cactagaaga aacacgcacg
      661 tgtacccaaa aatagagaca aacaaacaga caaaaataac aatgattcct accgactaaa
      721 gtaaaacaac actcacgtaa taaaaaatcc tagatacaac tcacttgtat tcctctcttc
      781 tttgaccatc actaaagctt ctctcttact atattcctca aataaaaaaa agttgcaatg
      841 attacttacc aatgtgccat aaactccgtg c
//
