LOCUS       sequence-127889-         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked natMX marker, integration into S. cerevisiae XI-1 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2375
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    165..198
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggactc cccatgcgag
       61 agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      121 gcccgggcta attatggggt gtcgccctta ttcgactcta tagtgaagtt cctattctct
      181 agaaagtata ggaacttctg aagtggggat ttaaatgcgg ccgcgctgag ggtttaatgg
      241 aatagtgacg ttgtgatgcg gtgagttcgg cggttagggg aatggtatat gataaaaaac
      301 ggaaacgtgc ttctttaatt taattgttta atattgttgc agatatataa aaagggggaa
      361 agaaccgaag atgtaattat ttttttatcg cctcaaccta aagcaagcaa taaggtataa
      421 agatcaggac gtctcgagcg ctgatatcta aatttgaagc cacgcaagta actacgtagg
      481 tcagagggga caaggaataa cacttgacat ttttcttttt tctttttttt tctttttttt
      541 ttttttgtta atcttggctt ctgtaccgta gcctcctgta tacggtttta gatgcagtat
      601 gtttatcatc gccgagaaat ttggggggtg caaaggaata aaatctgtgc tgaaatccgt
      661 gcaccgcatc aaactttctc ggaggattct ttgcagccga ttaagtcctc agcgagctcg
      721 catggaatgc gtgcgatgag cgacctc
//
