LOCUS       sequence-127896-         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked kanMX marker, integration into S. cerevisiae XI-2 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2224
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      95..284
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     protein_bind    332..365
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        420..763
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(831..850)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 tttaataata aaaatcataa atcataagaa attcgcttat ttagaagtgt caacaacgta
       61 tctaccaacg gaatgcgtgc gatcgcgtgc attcatccgc tctaaccgaa aaggaaggag
      121 ttagacaacc tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa
      181 cgttatttat atttcaaatt tttctttttt ttctgtacag acgcgtgtac gcatgtaaca
      241 ttatactgaa aaccttgctt gagaaggttt tgggacgctc gaagatcgcg tcagctgaag
      301 cttcgtacgc tgcaggtcga caacccttaa tataacttcg tataatgtat gctatacgaa
      361 gttattaggt ctagagatct gtttagcttg cctcgtcccc gccgggtcac ccggccagcg
      421 acatggaggc ccagaatacc ctccttgaca gtcttgacgt gcgcagctca ggggcatgat
      481 gtgactgtcg cccgtacatt tagcccatac atccccatgt ataatcattt gcatccatac
      541 attttgatgg ccgcacggcg cgaagcaaaa attacggctc ctcgctgcag acctgcgagc
      601 agggaaacgc tcccctcaca gacgcgttga attgtcccca cgccgcgccc ctgtagagaa
      661 atataaaagg ttaggatttg ccactgaggt tcttctttca tatacttcct tttaaaatct
      721 tgctaggata cagttctcac atcacatccg aacataaaca accatgggta aggaaaagac
      781 tcacgtttcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
      841 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga
      901 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg tt
//
