LOCUS       sequence-127897-         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked kanMX marker, integration into S. cerevisiae XI-2 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2224
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     78..95
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     79..95
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    565..598
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 ggaactgcca ggcatcaaac taagcagaag gcccctgacg gatggccttt ttgcgtttct
       61 acaaactctt tctgtgttgt aaaacgacgg ccagtcttaa gctcgggccc cctgggcggt
      121 tctgataacg agtaatcgtt aatccgcaaa taacgtaaaa acccgcttcg gcgggttttt
      181 ttatgggggg agtttaggga aagagcattt gtcagaatat ttaagggcgc ctgtcacttt
      241 gcttgatata tgagaattat ttaaccttat aaatgagaaa aaagcaacgc actttaaata
      301 agatacgttg ctttttcgat tgatgaacac ctataattaa actattcatc tattatttat
      361 gattttttgt atatacaata tttctagttt gttaaagaga attaagaaaa taaatctcga
      421 aaataataaa gggaaaatca gtttttgata tcaaaattat acatgtcaac gataatacaa
      481 aatataatac aaactataag atgttatcag tatttattat gcatttagaa taaattttgt
      541 gtcgccctta ttcgactcac tatagaagtt cctattctct agaaagtata ggaacttcac
      601 ttcattttat ttaaatttgc ggccgcgctg aggtcttaat gtgggaagat tccgctctac
      661 cagggaaaaa caaaattata tagaaaagaa ggtaagtaat aagtaatacg aaaaaaccca
      721 aaaaaaaaat tcaaataccc caaccctgtg cccttt
//
