LOCUS       sequence-127901-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked hphMX marker, integration into S. cerevisiae XI-3 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2195
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    189..222
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg
       61 tagtgtgggg actccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg
      121 ctcagtcgaa agactgggcc tttcgcccgg gctaattatg gggtgtcgcc cttattcgac
      181 tctatagtga agttcctatt ctctagaaag tataggaact tctgaagtgg ggatttaaat
      241 gcggccgcgc tgagggttta atagttactt gctctatgcg tttgcgcatc ctctttttac
      301 tttttttttt tcagtaaagc ctaagcataa atcgttttat acgtacgaca cgttcaactt
      361 ttcttggtta gtagtggcaa tctctgcaat acatacaggg agtcatggtc tatcatcttg
      421 tccaatcaaa gaagcatcgg ttcagatcga gcaaactgta gggagaaagg aaagtagaaa
      481 tgcagagtgt gctatatgtc caatctcggt tttgtagttt ggatgtcatt agagatctac
      541 cacccaaccg gctgctttca tgtggaacag aaaagaaatc ggggcgcttc ctcttctgta
      601 ttcctttagt taacgttttt attcagccat ctaaccatca tacccccata cggtaacaaa
      661 acctcttcta agaaaagaag tctctgctcc tccgccatct tatttttatt cgctgcgcgc
      721 gtttattgtc gcatcgctgg ccagcaaaaa attggttgcc tttttttacc taaaaaagac
      781 acatctaact gattagtttt ccgttttagg a
//
