LOCUS       sequence-127903-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression of pHoenix 
            (Synaptophysin-pHluorin-Arch3-mKate2) driven by human Synapsin I 
            promoter for optogenetic acidification of synaptic vesicles.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Rost BR, Schneider F, Grauel MK, Wozny C, G Bentz C, Blessing A, 
            Rosenmund T, Jentsch TJ, Schmitz D, Hegemann P, Rosenmund C
  TITLE     Optogenetic acidification of synaptic vesicles and lysosomes.
  JOURNAL   Nat Neurosci. 2015 Nov 9. doi: 10.1038/nn.4161.
  PUBMED    26551543
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2015 Nov 9. doi: 10.1038/nn.4161."
COMMENT     Sequence Label: pLenti-Synapsin-pHoenix-WPRE
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     586..614
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     regulatory      644..653
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctcttttcgt tgggatcttt cgaaagagta gaagttgtaa acaggtaatg gttgtctggt
       61 aaaaggacgg gcccatcgcc aattggagta ttttgttgat aatggtctgc tagttgaacg
      121 cctccatctt caatgttgtg tctaattttg aagttagctt tgattccatt cttttgtttg
      181 tctgccatga tgtacacctg gtgatcgtta tagttgtatt ccaatttgtg tccaagaatg
      241 tttccatctt ctttaaaatc aatacctttt aactcgattc tattaacaag ggtatcacct
      301 tcaaacttga cttcagcacg tgtcttgtag ttcccgtcat ctttgaaaaa tatagttctt
      361 tcctgtacat aaccttcggg catggcactc ttgaaaaagt catgccgttt catatgatct
      421 gggtatcttg aaaagcattg aacaccataa gttaaagtag tgacaagtgt tggccaagga
      481 acaggtagtt ttccagtagt gcaaataaat ttaagggtaa gttttccgta tgttgcatca
      541 ccttcaccct ctccactgac agaaaatttg tgcccattaa catcaccatc taattcaaca
      601 agaattggga caactccagt gaaaagttct tctcctttac ttcccatggt ggctagatcc
      661 atatctgtct ggcggcacat aggcatctcc ttgataatgt tctctgggtc agtggccatc
      721 ttcacatcgg acaggccttt ggcccaggcg gatgagctaa ctagccacat gaaagcgaac
      781 actgctgtgg ccaggaagtc catcattggc cctttgttgt tctctcggta
//
