LOCUS       sequence-127904-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked KlURA3 marker, integration into S. cerevisiae XII-1 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2191
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      70..259
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     protein_bind    307..340
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 aagaaattcg cttatttaga agtgtcaaca acgtatctac caacggaatg cgtgcgatcg
       61 cgtgcattca tccgctctaa ccgaaaagga aggagttaga caacctgaag tctaggtccc
      121 tatttatttt tttatagtta tgttagtatt aagaacgtta tttatatttc aaatttttct
      181 tttttttctg tacagacgcg tgtacgcatg taacattata ctgaaaacct tgcttgagaa
      241 ggttttggga cgctcgaaga tcgcgtcagc tgaagcttcg tacgctgcag gtcgacaacc
      301 cttaatataa cttcgtataa tgtatgctat acgaagttat taggtctaga gatcccaata
      361 caacagatca cgtgatcttt ttgtaagatg aagttgaagt agtgttgcac cgtgccaatg
      421 caggtggcta ttagattaaa tatgtgattt gttctattaa gtttcctgta taattaatgg
      481 ggagcgctga ttctcttttg gtacgcttcc catccagcat ttctgtatct ttcaccttca
      541 accttaggat ctctaccctt ggcgaaaagt cctctgccaa caatgatgat atctgatcca
      601 ccacttacaa cttcgtcgac ggttctgtac tgctgaccca atgcatcgcc tttgtcgtct
      661 aaacctacac ctggggtcat gattagccaa tcaaaccctt cttctcttcc tcccatatcg
      721 ttctgagcaa tgaacccaat aacgaaatct ttatcactct ttgcaatatc aacggtaccc
      781 ttagtatatt caccgtgtgc tagagaaccc ttggaagata attcagcaag ca
//
