LOCUS       sequence-127905-        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression of pHoenix 
            (Synaptophysin-pHluorin-Arch3-mKate2) driven by human Synapsin I 
            promoter for optogenetic acidification of synaptic vesicles.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Rost BR, Schneider F, Grauel MK, Wozny C, G Bentz C, Blessing A, 
            Rosenmund T, Jentsch TJ, Schmitz D, Hegemann P, Rosenmund C
  TITLE     Optogenetic acidification of synaptic vesicles and lysosomes.
  JOURNAL   Nat Neurosci. 2015 Nov 9. doi: 10.1038/nn.4161.
  PUBMED    26551543
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2015 Nov 9. doi: 10.1038/nn.4161."
COMMENT     Sequence Label: pLenti-Synapsin-pHoenix-WPRE
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..812
                     /codon_start=1
                     /product="light-driven proton pump archaerhodopsin-3 from 
                     Halorubrum sodomense (Chow et al., 2010)"
                     /label=Arch
                     /translation="MDPIALQAGYDLLGDGRPETLWLGIGTLLMLIGTFYFLVRGWGVT
                     DKDAREYYAVTILVPGIASAAYLSMFFGIGLTEVTVGGEMLDIYYARYADWLFTTPLLL
                     LDLALLAKVDRVTIGTLVGVDALMIVTGLIGALSHTAIARYSWWLFSTICMIVVLYFLA
                     TSLRSAAKERGPEVASTFNTLTALVLVLWTAYPILWIIGTEGAGVVGLGIETLLFMVLD
                     VTAKVGFGFILLRSRAILGDTEAPEPSAGADVSAAD"
ORIGIN
        1 atggcatgga tgaactatac aaaaccggta ccggatccat ggaccccatc gctctgcagg
       61 ctggttacga cctgctgggt gacggcagac ctgaaactct gtggctgggc atcggcactc
      121 tgctgatgct gattggaacc ttctactttc tggtccgcgg atggggagtc accgataagg
      181 atgcccggga atattacgct gtgactatcc tggtgcccgg aatcgcatcc gccgcatatc
      241 tgtctatgtt ctttggtatc gggcttactg aggtgaccgt cgggggcgaa atgttggata
      301 tctattatgc caggtacgcc gactggctgt ttaccacccc acttctgctg ctggatctgg
      361 cccttctcgc taaggtggat cgggtgacca tcggcaccct ggtgggtgtg gacgccctga
      421 tgatcgtcac tggcctcatc ggagccttga gccacacggc catagccaga tacagttggt
      481 ggttgttctc tacaatttgc atgatagtgg tgctctattt tctggctaca tccctgcgat
      541 ctgctgcaaa ggagcggggc cccgaggtgg catctacctt taacaccctg acagctctgg
      601 tcttggtgct gtggaccgct taccctatcc tgtggatcat aggcactgag ggcgctggcg
      661 tggtgggcct gggcatcgaa actctgctgt ttatggtgtt ggacgtgact gccaaggtcg
      721 gctttggctt tatcctgttg agatcccggg ctattctggg cgacaccgag gcaccagaac
      781 ccagtgccgg tgccgatgtc agtgccgccg accgtacgat ggtgagcgag ctgattaagg
      841 agaacatgca catgaagctg tacatggagg gcaccgtgaa caaccaccac ttcaagtgca
      901 catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggcggtcg
      961 agggcggccc tctccccttc gccttcgaca tcctggctac cagcttcatg tacggcagca
     1021 aaaccttcat caaccacacc cagggcatcc c
//
