LOCUS       sequence-127907-         624 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked KlURA3 marker, integration into S. cerevisiae XII-1 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2191
FEATURES             Location/Qualifiers
     source          1..624
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    482..515
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 acgacggcca gtcttaagct cgggccccct gggcggttct gataacgagt aatcgttaat
       61 ccgcaaataa cgtaaaaacc cgcttcggcg ggttttttta tggggggagt ttagggaaag
      121 agcatttgtc agaatattta agggcgcctg tcactttgct tgatatatga gaattattta
      181 accttataaa tgagaaaaaa gcaacgcact ttaaataaga tacgttgctt tttcgattga
      241 tgaacaccta taattaaact attcatctat tatttatgat tttttgtata tacaatattt
      301 ctagtttgtt aaagagaatt aagaaaataa atctcgaaaa taataaaggg aaaatcagtt
      361 tttgatatca aaattataca tgtcaacgat aatacaaaat ataatacaaa ctataagatg
      421 ttatcagtat ttattatgca tttagaataa attttgtgtc gcccttattc gactcactat
      481 agaagttcct attctctaga aagtatagga acttcacttc attttattta aatttgcggc
      541 cgcgctgagg tcttaatcaa tcctcgcatt tcagcttcca ttatatttga tgactgtttc
      601 tcaatcttta tgttatcttc ttac
//
