LOCUS       sequence-127908-         987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression of pHoenix 
            (Synaptophysin-pHluorin-Arch3-mKate2) driven by human Synapsin I 
            promoter for optogenetic acidification of synaptic vesicles.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Rost BR, Schneider F, Grauel MK, Wozny C, G Bentz C, Blessing A, 
            Rosenmund T, Jentsch TJ, Schmitz D, Hegemann P, Rosenmund C
  TITLE     Optogenetic acidification of synaptic vesicles and lysosomes.
  JOURNAL   Nat Neurosci. 2015 Nov 9. doi: 10.1038/nn.4161.
  PUBMED    26551543
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2015 Nov 9. doi: 10.1038/nn.4161."
COMMENT     Sequence Label: pLenti-Synapsin-pHoenix-WPRE
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atctttcaca aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattcta
       61 gagtcgcggc cttaattaat tacatctgat tggagaagga ggtgggcgca ccctgttggc
      121 cgtagccttg ctgcccatag tcgccctgag gcccgtagcc accgccaccg ccgctggctg
      181 gctgcccgta atcgggttga taaccaccct gaggcccgta ggagtcctgg ggcccatagc
      241 ctccggggcc ctgcccatag cccgcatcgc cgtaggcatc gccaggagct ggttgctttt
      301 ctggggcgcc tggaggtgcg cgcatgaatg gggcggccca gcctgtctcc ttgaacacga
      361 accataggtt gccaacccag agcaccaggt tcaggaagcc aaacaccacc gaggtgttga
      421 gtcctgaagt cacagggtcc ctcagttcct tgcatgtgat tcgagctcca ccggtggtgt
      481 ttaaaccaga gctgttttca gagatgttgt aggaaatctg cagccctctt tccccataca
      541 catccggtct caaggttacc cccggtgact ttaactggtc ctggtagtcg ggggtgtagg
      601 gatcaatggt ctgcatcagc acatagatgc acaaggcaaa gagcccagtc atgaccacgt
      661 agaaagctgc atagtacagg ctgatccaca cccaccgggc tggggtgcgg cccagcatct
      721 gcccagtgtc cgggttccaa cagtattgcc ggaattcggc catgcgctgg ctgcatgact
      781 tcttctcctg cagggctgca agctttctgt gccccagttt gctagggagg tcgcagtatc
      841 tggccacagc cacctcgtgc tgctcgacgt aggtctcttt gtcggcctcc ttgattcttt
      901 ccagtcttct gtccacatag tagacgccgg gcatcttgag gttcttagcg ggtttcttgg
      961 atctgtatgt ggtcttcaag ttgcaga
//
