LOCUS       sequence-127916-         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked KlLEU2 marker, integration into S. cerevisiae XII-2 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2192
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      78..267
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     protein_bind    315..348
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 taaatcataa gaaattcgct tatttagaag tgtcaacaac gtatctacca acggaatgcg
       61 tgcgatcgcg tgcattcatc cgctctaacc gaaaaggaag gagttagaca acctgaagtc
      121 taggtcccta tttatttttt tatagttatg ttagtattaa gaacgttatt tatatttcaa
      181 atttttcttt tttttctgta cagacgcgtg tacgcatgta acattatact gaaaaccttg
      241 cttgagaagg ttttgggacg ctcgaagatc gcgtcagctg aagcttcgta cgctgcaggt
      301 cgacaaccct taatataact tcgtataatg tatgctatac gaagttatta ggtctagaga
      361 tccgcaggct aaccggaacc tgtattattt agtttatgct acgttaaata aagacctttc
      421 gttcacataa ctgaatgtgt aatggccttg agatttcaag cataccaagt tggtggagac
      481 ggggtcgtta caaaagactc tttaagccaa gatttccttg acagccttgg cgatagcatc
      541 gccaacctca gtggtagagt tagaaccacc aaggtcaccg gttctgacac ctgcatccaa
      601 gacatttcta acagcttctt caagagccct accttcttca accaaatcca aggataactt
      661 caacatcata gctgcagata agatggtagc aattgggtta accttgtttg ctggtaaatc
      721 tggggcagaa ccatgacatg gttcgtacaa accgaatgcc ttgttagtgt caggtaggga
      781 agctagagat gcagaaggta ataaacccaa agaacctgga ataacagagg cttcatcgga
      841 gataatatca ccaaacatgt tgttggtaat aacaacaccg tttagcttag ttggtgattt
      901 aaccaaaatc atagcagcag agtcgatcaa ttggtgctga acagttaatt gtgggaactc
      961 agtcttga
//
