LOCUS       sequence-127920-         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked KlLEU2 marker, integration into S. cerevisiae XII-2 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2192
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    186..219
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 ggtggtccca cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag
       61 tgtggggact ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc
      121 agtcgaaaga ctgggccttt cgcccgggct aattatgggg tgtcgccctt attcgactct
      181 atagtgaagt tcctattctc tagaaagtat aggaacttct gaagtgggga tttaaatgcg
      241 gccgcgctga gggtttaatc ggcatgcaaa catctacaca attagcaagg gcaatccata
      301 ttttgtcttt tcgcgccctg gaaaggccta agtaatgtcg taaacgcatt ctatctgtac
      361 ttcaactctc ctctgtgcat tggtttgtgc aaatcacatt ttacgatact gccagatata
      421 tgcaaaaaga gaaaaccaag ggaccagaac aaagcaaaat tacgatattc ttcgaattcc
      481 ttcgtgcttg actaagacaa agggatggac gtagcgattt ttagcgggcc aagaactggt
      541 tccgaaaaag cacaggtaca ccgaaccctc agctaaggag ggacagcacc gatgcggaag
      601 gacaaacttt ctttttgcct atcacagtat cttatcgagc taactatttt cgacacacat
      661 gaaaaagcag aaatattaac gaaaaagaaa agaaagacca tgtcatgtac gggcaatcag
      721 aatctgtaac aagcgccatt tttttttctg tatcgggccc tccttactgc tctccttccg
      781 tgtaacgcgt tatgattaag tcctcagcga gctcgcatgg aatgcgtgcg atgagcgacc
      841 tcatgctata cctgagaaag caacctgacc tacaggaaag agttactcaa gaataagaat
      901 tttcgtttta aaacctaa
//
