LOCUS       sequence-127921-         663 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the Gal4 Binding Domain fused to the BDR Transcriptional 
            Repressor as a level 0 GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pGal4BD::BDR (GB0734)
FEATURES             Location/Qualifiers
     source          1..663
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      390..399
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      394..403
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(433..452)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(434..452)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(459..476)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(459..475)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(468..490)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 atcacgcgtt gggagctctc ccatatggtc gacctgcagg cggccgcgaa ttcactagtg
       61 attgggcgat gagtggtctc gaagctcaac attccatatt aacaccaaaa agtctaagag
      121 tcttagaatt acccctagga aaaatcagta gaaatagctg ttccagtctt tctagccttg
      181 attccacttc tgtcagatgt gccctagtca gcggagatct tttggttttg ggagagtagc
      241 gacactccca gttgttcttc agacacttgg cgcacttcgg tttttctttg gagcacttga
      301 gctttttaag tcggcaaata tcgcatgctt gttcgataga agacagtagc ttcattcgag
      361 accactcatc gcccaatcga attcccgcgg ccgccatggc ggccgggagc atgcgacgtc
      421 gggcccaatt cgccctatag tgagtcgtat tacaattcac tggccgtcgt tttacaacgt
      481 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
      541 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
      601 ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
      661 cac
//
