LOCUS       sequence-127923-         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked KlLEU2 marker, integration into S. cerevisiae XII-2 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2192
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     70..87
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     71..87
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    557..590
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 caggcatcaa actaagcaga aggcccctga cggatggcct ttttgcgttt ctacaaactc
       61 tttctgtgtt gtaaaacgac ggccagtctt aagctcgggc cccctgggcg gttctgataa
      121 cgagtaatcg ttaatccgca aataacgtaa aaacccgctt cggcgggttt ttttatgggg
      181 ggagtttagg gaaagagcat ttgtcagaat atttaagggc gcctgtcact ttgcttgata
      241 tatgagaatt atttaacctt ataaatgaga aaaaagcaac gcactttaaa taagatacgt
      301 tgctttttcg attgatgaac acctataatt aaactattca tctattattt atgatttttt
      361 gtatatacaa tatttctagt ttgttaaaga gaattaagaa aataaatctc gaaaataata
      421 aagggaaaat cagtttttga tatcaaaatt atacatgtca acgataatac aaaatataat
      481 acaaactata agatgttatc agtatttatt atgcatttag aataaatttt gtgtcgccct
      541 tattcgactc actatagaag ttcctattct ctagaaagta taggaacttc acttcatttt
      601 atttaaattt gcggccgcgc tgaggtctta atgagcgaac gtaagagagg ttaatgtcct
      661 ctaactatca gtccaatgac agtattttct ccttctcaca aactacaata aatatctacc
      721 aatctctttt cgaataagga ctaatgatat acgctagttc ctgtttggta aatgaaggct
      781 agtgttcgag aatggagaac ttcgtagtcg gttaaaacag ctgtagtgtt ctggccttct
      841 tagaaagaat aaatagttca ggagtaaaat caaaatatct tttaatataa actaagacaa
      901 agaaagaaac aagaattttg atctgaaaaa atcgaaaaaa aggaacgaat ggatcgatga
      961 aaattttcga gactaggtta taatt
//
