LOCUS       sequence-127924-         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked SpHIS5 marker, integration into S. cerevisiae XII-5 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2336
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      76..265
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     protein_bind    313..346
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        401..744
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 aatcataaga aattcgctta tttagaagtg tcaacaacgt atctaccaac ggaatgcgtg
       61 cgatcgcgtg cattcatccg ctctaaccga aaaggaagga gttagacaac ctgaagtcta
      121 ggtccctatt tattttttta tagttatgtt agtattaaga acgttattta tatttcaaat
      181 ttttcttttt tttctgtaca gacgcgtgta cgcatgtaac attatactga aaaccttgct
      241 tgagaaggtt ttgggacgct cgaagatcgc gtcagctgaa gcttcgtacg ctgcaggtcg
      301 acaaccctta atataacttc gtataatgta tgctatacga agttattagg tctagagatc
      361 tgtttagctt gcctcgtccc cgccgggtca cccggccagc gacatggagg cccagaatac
      421 cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat
      481 ttagcccata catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc
      541 gcgaagcaaa aattacggct cctcgctgca gacctgcgag cagggaaacg ctcccctcac
      601 agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt
      661 gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca
      721 catcacatcc gaacataaac aaccatgggt aggagggctt ttgtagaaag aaatacgaac
      781 gaaacgaaaa tcagcgttgc catcgctttg gacaaagctc ccttacctga agagtcgaat
      841 tttattgatg aacttataac ttccaagcat gcaaaccaaa aggg
//
