LOCUS       sequence-127926-         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked SpHIS5 marker, integration into S. cerevisiae XII-5 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2336
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..70
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     54..70
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    540..573
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 agaaggcccc tgacggatgg cctttttgcg tttctacaaa ctctttctgt gttgtaaaac
       61 gacggccagt cttaagctcg ggccccctgg gcggttctga taacgagtaa tcgttaatcc
      121 gcaaataacg taaaaacccg cttcggcggg tttttttatg gggggagttt agggaaagag
      181 catttgtcag aatatttaag ggcgcctgtc actttgcttg atatatgaga attatttaac
      241 cttataaatg agaaaaaagc aacgcacttt aaataagata cgttgctttt tcgattgatg
      301 aacacctata attaaactat tcatctatta tttatgattt tttgtatata caatatttct
      361 agtttgttaa agagaattaa gaaaataaat ctcgaaaata ataaagggaa aatcagtttt
      421 tgatatcaaa attatacatg tcaacgataa tacaaaatat aatacaaact ataagatgtt
      481 atcagtattt attatgcatt tagaataaat tttgtgtcgc ccttattcga ctcactatag
      541 aagttcctat tctctagaaa gtataggaac ttcacttcat tttatttaaa tttgcggccg
      601 cgctgaggtc ttaatctgcg ataccttttg tgatggctaa acaaacagac atctttttat
      661 atgtttttac ttctgtatat cgtgaagtag taagtgataa gcgaatttgg ctaagaacgt
      721 tgtaagtgaa caagggacct cttttgcctt tcaaaaaagg attaaataga gttaatcatt
      781 gagatttagt tttcgttaga ttc
//
