LOCUS       sequence-127927-         611 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked SpHIS5 marker, integration into S. cerevisiae XII-5 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2336
FEATURES             Location/Qualifiers
     source          1..611
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    140..173
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 agcgccgatg gtagtgtggg gactccccat gcgagagtag ggaactgcca ggcatcaaat
       61 aaaacgaaag gctcagtcga aagactgggc ctttcgcccg ggctaattat ggggtgtcgc
      121 ccttattcga ctctatagtg aagttcctat tctctagaaa gtataggaac ttctgaagtg
      181 gggatttaaa tgcggccgcg ctgagggttt aatcaatctg gcggcttgag ttctcaacat
      241 gttttatttt ttacttatat tgctggtagg gtaaaaaaat ataactccta ggaataggtt
      301 gtctatatgt ttttgtcttg cttctataat tgtaacaaac aaggaaaggg aaaatactgg
      361 gtgtaaaagc cattgagtca agttaggtca tcccttttat acaaaatttt tcaatttttt
      421 ttccaagatt cttgtacgat taattatttt ttttttgcgt cctacagcgt gatgaaaatt
      481 tcgcctgctg caagatgagc gggaacgggc gaaatgtgca cgcgcacaac ttacgaaacg
      541 cggatgagtc actgacagcc accgcagagg ttctgactcc tactgagctc tattggaggt
      601 ggcagaaccg g
//
